Operating Sunday through Friday, the zoo train route is circular, giving a general overview of the zoo grounds. The train engineer provides explanations along the way, usually in Hebrew.

The zoo train is intended, among other things, to give a general overview of the zoo, to provide amusement for children, and to assist people who have difficulty walking. For your own enjoyment, we strongly recommend that you walk through the zoo on foot. The animals should be seen from close up, so that you can view them properly, and see them eat, rest, and play in their roomy enclosures.
The train does not operate on Saturdays and Holidays, nor does it run on the Intermediate Days (Hol HaMo'ed) of the Pesach and Succot Holidays.
For the sake of public safety, the zoo management reserves the right to halt train service on days when the zoo is particularly crowded.

Starting Point: At the far end of the bridge, on the lower level
First Station: (Only for getting off) Next to the Bear Exhibit
Second Station: (For boarding and getting off) Next to the Noah's Ark Visitors' Center and the exit of the Bible Land Wildlife Preserve
Third Station: (For boarding and getting off) Next to the Hippopotamus Exhibit and opposite
the Elephant Exhibit
Final Station: (Only for getting off) Adjacent to the Flamingo Pond, on the lower level